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\n \n\n \n \n Kawada, T., Shiraishi, A., Matsubara, S., Hozumi, A., Horie, T., Sasakura, Y., & Satake, H.\n\n\n \n \n \n \n \n Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes.\n \n \n \n \n\n\n \n\n\n\n Frontiers in Endocrinology, 12: 668564. May 2021.\n \n\n\n\n
\n\n\n\n \n \n \"VasopressinPaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n  \n \n abstract \n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{kawada_vasopressin_2021,\n\ttitle = {Vasopressin {Promoter} {Transgenic} and {Vasopressin} {Gene}-{Edited} {Ascidian}, \\textit{{Ciona} intestinalis} {Type} {A} (\\textit{{Ciona} robusta}): {Innervation}, {Gene} {Expression} {Profiles}, and {Phenotypes}},\n\tvolume = {12},\n\tissn = {1664-2392},\n\tshorttitle = {Vasopressin {Promoter} {Transgenic} and {Vasopressin} {Gene}-{Edited} {Ascidian}, {Ciona} intestinalis {Type} {A} ({Ciona} robusta)},\n\turl = {https://www.frontiersin.org/articles/10.3389/fendo.2021.668564/full},\n\tdoi = {10.3389/fendo.2021.668564},\n\tabstract = {Oxytocin (OT) and vasopressin (VP) superfamily neuropeptides are distributed in not only vertebrates but also diverse invertebrates. However, no VPergic innervation of invertebrates has ever been documented. In the ascidian,\n              Ciona intestinalis\n              Type A (\n              Ciona robusta\n              ), an OT/VP superfamily peptide was identified, and the\n              Ciona\n              vasopressin (CiVP) induces oocyte maturation and ovulation. In the present study, we characterize the innervation and phenotypes of genetically modified\n              Ciona\n              :\n              CiVP\n              promoter-Venus transgenic and\n              CiVP\n              mutants.\n              CiVP\n              promoter-Venus transgenic\n              Ciona\n              demonstrated that\n              CiVP\n              gene was highly expressed in the cerebral ganglion and several nerves. Fluorescence was also detected in the ovary of young\n              CiVP\n              promoter-Venus transgenic ascidians, suggesting that the\n              CiVP\n              gene is also expressed temporarily in the ovary of young ascidians. Furthermore, a marked decrease of post-vitellogenic (stage III) follicles was observed in the ovary of\n              CiVP\n              mutants, whereas pre-vitellogenic (stage I) and vitellogenic (stage II) follicles were increased in the mutant ovary, compared with that of wildtype\n              Ciona\n              . Gene expression profiles showed that the expression of various genes, including genes related to ovarian follicle growth, was altered in the ovary of\n              CiVP\n              mutants. Altogether, these results indicated that CiVP, mainly as a neuropeptide, plays pivotal roles in diverse biological functions, including growth of early-stage ovarian follicles\n              via\n              regulation of the expression of a wide variety of genes. This is the first report describing a\n              VP\n              gene promoter-transgenic and\n              VP\n              gene-edited invertebrate and also on its gene expression profiles and phenotypes.},\n\turldate = {2021-08-06},\n\tjournal = {Frontiers in Endocrinology},\n\tauthor = {Kawada, Tsuyoshi and Shiraishi, Akira and Matsubara, Shin and Hozumi, Akiko and Horie, Takeo and Sasakura, Yasunori and Satake, Honoo},\n\tmonth = may,\n\tyear = {2021},\n\tpages = {668564},\n}\n\n
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\n Oxytocin (OT) and vasopressin (VP) superfamily neuropeptides are distributed in not only vertebrates but also diverse invertebrates. However, no VPergic innervation of invertebrates has ever been documented. In the ascidian, Ciona intestinalis Type A ( Ciona robusta ), an OT/VP superfamily peptide was identified, and the Ciona vasopressin (CiVP) induces oocyte maturation and ovulation. In the present study, we characterize the innervation and phenotypes of genetically modified Ciona : CiVP promoter-Venus transgenic and CiVP mutants. CiVP promoter-Venus transgenic Ciona demonstrated that CiVP gene was highly expressed in the cerebral ganglion and several nerves. Fluorescence was also detected in the ovary of young CiVP promoter-Venus transgenic ascidians, suggesting that the CiVP gene is also expressed temporarily in the ovary of young ascidians. Furthermore, a marked decrease of post-vitellogenic (stage III) follicles was observed in the ovary of CiVP mutants, whereas pre-vitellogenic (stage I) and vitellogenic (stage II) follicles were increased in the mutant ovary, compared with that of wildtype Ciona . Gene expression profiles showed that the expression of various genes, including genes related to ovarian follicle growth, was altered in the ovary of CiVP mutants. Altogether, these results indicated that CiVP, mainly as a neuropeptide, plays pivotal roles in diverse biological functions, including growth of early-stage ovarian follicles via regulation of the expression of a wide variety of genes. This is the first report describing a VP gene promoter-transgenic and VP gene-edited invertebrate and also on its gene expression profiles and phenotypes.\n
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\n \n\n \n \n Onuma, T. A., Nakanishi, R., Sasakura, Y., & Ogasawara, M.\n\n\n \n \n \n \n \n Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.\n \n \n \n \n\n\n \n\n\n\n Developmental Biology, 477: 219–231. September 2021.\n \n\n\n\n
\n\n\n\n \n \n \"Nkx2-1Paper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{onuma_nkx2-1_2021,\n\ttitle = {Nkx2-1 and {FoxE} regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate \\textit{{Oikopleura} dioica}},\n\tvolume = {477},\n\tissn = {00121606},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S0012160621001421},\n\tdoi = {10.1016/j.ydbio.2021.05.021},\n\tlanguage = {en},\n\turldate = {2021-08-06},\n\tjournal = {Developmental Biology},\n\tauthor = {Onuma, Takeshi A. and Nakanishi, Rina and Sasakura, Yasunori and Ogasawara, Michio},\n\tmonth = sep,\n\tyear = {2021},\n\tpages = {219--231},\n}\n\n
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\n \n\n \n \n Sasakura, Y., & Harvey, B. P.\n\n\n \n \n \n \n \n Institute Profile: Shimoda Marine Research Center, University of Tsukuba.\n \n \n \n \n\n\n \n\n\n\n Limnology and Oceanography Bulletin, 30(3): 116–118. August 2021.\n \n\n\n\n
\n\n\n\n \n \n \"InstitutePaper\n  \n \n\n \n \n doi\n  \n \n\n \n link\n  \n \n\n bibtex\n \n\n \n\n \n\n \n \n \n \n \n \n \n\n  \n \n \n\n\n\n
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@article{sasakura_institute_2021,\n\ttitle = {Institute {Profile}: {Shimoda} {Marine} {Research} {Center}, {University} of {Tsukuba}},\n\tvolume = {30},\n\tissn = {1539-607X, 1539-6088},\n\tshorttitle = {Institute {Profile}},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/lob.10457},\n\tdoi = {10.1002/lob.10457},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2021-12-21},\n\tjournal = {Limnology and Oceanography Bulletin},\n\tauthor = {Sasakura, Yasunori and Harvey, Ben P.},\n\tmonth = aug,\n\tyear = {2021},\n\tpages = {116--118},\n}\n\n
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